Extraction of nuclear proteins with increased DNA binding activity.
نویسندگان
چکیده
We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number of samples for gel shift analysis.
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ورودعنوان ژورنال:
- BioTechniques
دوره 28 5 شماره
صفحات -
تاریخ انتشار 2000